
( Brand: Leica ), ( Model: ET-DAPI ), ( Part Type: Filter ), ( Manufacturer Part Number: 232FL4900072 )
The LEICA 232FL4-900072 Microscope Filter Cube ET-DAPI is a high-performance filter set designed for use with fluorescence microscopes. This filter cube is specifically engineered for excitation and emission of the DAPI (4',6-diamidino-2-phenylindole) fluorochrome, which is commonly used for staining nuclear DNA in various applications, including cell biology and histology.
The ET-DAPI filter cube comprises a set of precision-crafted glass filter elements that ensure excellent optical performance and consistent fluorescence signal. The DAPI excitation filter (ET525/50x) is designed to optimally excite DAPI fluorescence by transmitting light in the range of 460 to 490 nm. The DAPI emission filter (ET590/40m) is engineered to efficiently collect and transmit the fluorescence emitted by DAPI, with a central wavelength of 590 nm and a bandpass of 40 nm.
The filter cube also includes a dichroic mirror that reflects excitation light and transmits emission light, ensuring that the excitation light does not interfere with the observation of the fluorescence signal. The filter cube is enclosed in a robust metal housing, which provides both mechanical protection and shielding from external light.
The LEICA 232FL4-900072 Microscope Filter Cube ET-DAPI is compatible with a wide range of fluorescence microscopes and is an essential tool for researchers and technicians working in fields such as molecular biology, cell biology, and histology. With its superior optical performance and rugged construction, this filter cube is an indispensable component of any advanced microscopy setup.
In summary, the LEICA 232FL4-900072 Microscope Filter Cube ET-DAPI is a high-quality filter set designed for excitation and emission of the DAPI fluorochrome. It consists of precision-crafted glass filter elements, a dichroic mirror, and a robust metal housing, making it an essential tool for researchers and technicians working in various fields of microscopy. The filter cube optimally excites and collects DAPI fluorescence, ensuring excellent signal and consistent results.
The Leica ET-DAPI filter cube (232FL4900072) is a popular choice for fluorescence microscopy applications, particularly for DAPI (4',6-diamidino-2-phenylindole) staining. DAPI is commonly used for counterstaining nucleic acids, allowing the visualization of cellular nuclei. Let's discuss the pros and cons of purchasing this filter cube.
Pros:1. High-quality optics: Leica is known for its superior optical components. The ET-DAPI filter cube offers excellent light transmission and minimal chromatic aberrations, ensuring crisp images and accurate data.
2. Wide excitation/emission bands: The filter cube has a broad excitation band (350-385 nm) and a narrow emission band (420-480 nm), which allows for the detection of weak DAPI signals.
3. Easy installation: The filter cube can be easily installed and removed from the microscope, making it a flexible solution for various applications.
4. Durable construction: The filter cube is robustly built and designed to withstand rigorous use in a laboratory setting.
Cons:1. Cost: The Leica ET-DAPI filter cube is more expensive than some other options on the market. This might be a significant consideration for researchers or institutions with limited budgets.
2. Limited functionality: This filter cube is designed specifically for DAPI staining applications. If you require a more versatile filter cube for other fluorescence markers, you might need to invest in additional filter cubes.
Conclusion:The Leica ET-DAPI filter cube (232FL4900072) offers excellent optical quality, wide excitation/emission bands, easy installation, and durable construction. However, its high cost might be a limiting factor for some researchers or institutions. If you primarily work with DAPI staining and require the best possible results, this filter cube is an excellent investment. For those with more diverse fluorescence requirements or limited budgets, alternative options may be worth considering.
Recommendation:If you prioritize high-quality results for DAPI staining applications and have the budget for it, the Leica ET-DAPI filter cube (232FL4900072) is an excellent choice. For researchers or institutions with limited budgets or more diverse fluorescence requirements, exploring alternative filter cube options from other reputable manufacturers (such as Nikon, Zeiss, or Olympus) may be a more cost-effective solution.
Good condition with signs of normal use - see pictures. Only comes with what is pictured, nothing else.